Tuesday 24th May, 2022. The Gen 2 range has grown again, with the addition of qEVsingle Gen 2 columns.
Optimised for a 150 µL loading volume, qEVsingle Legacy columns are widely used for the size-based separation of low-volume biological samples.
Now, EV researchers can benefit from the enhanced separation provided by qEVsingle columns made using Izon’s high-performing, proprietary Gen 2 resin.
As has been established for other columns in the range, including the qEVoriginal, qEV1, and qEV10, samples separated using the qEVsingle Gen 2 show far better resolution than their predecessors, the Legacy columns.
The enhanced resolution of Gen 2 columns is an advantage for the separation of soluble, contaminating protein from extracellular vesicles (EVs) and similarly sized particles; depending on the downstream analysis of choice, researchers may wish to alter the collected volumes in a way that changes EV-protein proportions and subsequently prioritises EV concentration, EV recovery, or EV purity.
Overall, EV-containing volumes can be obtained with higher purity (Figure 1 and 2) without compromising EV concentration (Figure 3).
As the only column designed for single use, the qEVsingle is commonly used for RNA-based studies. Like all qEV columns, qEVsingle columns are available in two types: the 35 nm and 70 nm series, which have different optimum recovery ranges (35 – 350 nm and 70 – 1000 nm, respectively).
Together with the Automatic Fraction Collector, qEV columns deliver rapid, precise and consistent separation of extracellular vesicles – important considerations for reproducible research and the scale-up of EV-based diagnostic tests.