Streamline Your Extracellular Vesicle Isolation

Extracellular vesicles (EVs) are shaping our understanding of cell biology and offer exciting opportunities for biomarker and therapeutic development. But without reproducible and scalable EV isolation, progress in this field is restricted. qEV columns harness size exclusion chromatography to give you highly purified and functional EVs, without the laborious and irreproducible nature inherent to other techniques. Accelerated further by qEV instrumentation, the nano-world is yours to unlock.

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image of the AFC v2
Isolate EVs in minutes, not hours

Forget centrifugation marathons - EVs elute from qEV columns within 15 minutes.

Simple & reproducible

Consistently and easily separate EVs from soluble proteins with qEV columns. Combine with the Automatic Fraction Collector to enhance reproducibility and ease of use.

A gentle approach to EV isolation

qEV columns offer a gentle approach to isolation, allowing EVs to remain intact.

graph
Figure 1.

Eluted protein and extracellular vesicles (EVs) and similarly sized particles >60 nm in human plasma (0.5 mL loading volume) separated on qEVoriginal/35 nm Gen 2 and qEVoriginal/70 nm Gen 2 columns. EV concentration was measured using an Exoid and protein levels by bicinchoninic acid (BCA) assay. Faded bars represent calculated individual concentrations based off pooled sample measurements.*Volumes are labelled as the highest volume in that sample i.e label “0.4” refers to the volume from 0.0-0.4 mL after the buffer volume, label “0.8” refers to the volume from 0.4-0.8 mL after the buffer volume and so on.

Harness SEC to Separate EVs From Contaminants

qEV columns use principles of size exclusion chromatography (SEC) to separate exosomes and other extracellular vesicles (EVs) from contaminating particles, such as proteins and lipoproteins. Available in a growing range of sizes and types, qEV columns cater to the broad spectrum of samples under investigation across the EV field. With Gen 2 columns (35 nm & 70 nm series) removing approximately 99% of soluble protein, qEV isolation is favoured largely for its EV isolate purity. The strength of SEC also lies in its versatility, offering EV applications a scalable way forward.

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How qEV Isolation Columns Work

qEV isolation is based on principles of size exclusion chromatography, a method in which molecules and particles in solution are separated by their size as they pass through a column consisting of porous resin particles. In qEV isolation, larger particles are unable to enter pores in the resin, and instead flow through the resin relatively quickly. In contrast, particles smaller than the lower end of the isolation range (20, 35 nm or 70 nm) enter pores in the resin, which slows their journey. Through this size-based separation, EVs and soluble protein are separated to a high degree of resolution, and a purified volume can be obtained.

qEV Solutions Enabling Automated & Scalable EV Isolation

Extracellular vesicle (EV) isolation has traditionally been a tedious process. We are here to change that, dispelling the myth that ‘size exclusion chromatography (SEC) isn’t scalable’. Our range of qEV columns demonstrates this beautifully – our smallest can handle volumes as tiny as 150 µL, while our largest customised column is almost too big to hug! In addition, our qEV instrumentation and services introduce a new level of automation and scalability in EV isolation. The Automatic Fraction Collector (AFC) facilitates precise and reproducible isolation, streamlining the process of collecting a purified EV isolate.  With qEV PurePath for Therapeutics, large-scale columns are optimised and built into wider bioprocessing workflows. With qEV PurePath for Diagnostics, we can help you move towards more high-throughput settings, bringing your EV biomarker to life.

Learn more about the AFC

Introducing qEV Gen 2

The new range of qEV columns are made with an agarose resin, which delivers a more purified extracellular vesicle (EV)-containing eluate. The release of Gen 2 qEV columns are in line with the need to support the rapidly growing areas of EV research and applications, where sample purity has a huge impact on results downstream.

Learn more about qEV Gen 2
graph
Figure 3.

Legacy vs Gen 2. Data shown for a human plasma sample collected on qEV original columns, 0.5 mL loading volume.

Step 1 / Choose Your qEV Series

There are three series of qEV columns, each of which differ in particle size recovered, purity, and yield. Which is the best qEV series for you? That depends on your research question, the size of your particles of interest, and your tolerance for EV isolate purity.

20nm transparent

20 nm series

Exceptional particle yield.
Isolate particles 20 nm and above.
Sample purity is slightly compromised, with more protein and lipoprotein present in purified isolates.

35nm gen 2 blue

35 nm series

The middle ground, bringing balanced performance.
Isolate particles 35 nm and above.
Provides a compromise between yield and purity.

35nm gen2 magenta

70 nm series

Exceptional purity.
Isolate particles 70 nm and above, with slightly lower yield.
Delivers exceptionally pure isolates, with minimal protein and lipoprotein.

Step 2 / Choose Your Column Size

Column size selection is based on the sample loading volume required. Each column has a sample loading volume recommended for highest purity. If you are not sure which column is the right size, please contact the Izon team.

Izon products are designed and manufactured under a quality system certified to ISO 13485:2016.

Kits for Concentrating EVs and Extracting RNA

Impactful extracellular vesicle (EV) research and the development of downstream applications are only possible when supported by technologies that standardise and maximise the purification of EV and EV-RNA samples.

To support your downstream applications, Izon offers several kits for concentrating EVs and extracting EV-RNA, post qEV isolation.

Learn more about the qEV kits
RNA Extraction Kit

Natalie Turner, PhD Student at Queensland University of Technology

"Izon’s qEV columns offer a simple, effective and reproducible way of purifying EVs directly from biofluids or from EV enriched samples following differential centrifugation. We use the qEV 70 nm legacy (original) columns for all of our EV preps from plasma and milk samples, which undergo downstream processing for mass spectrometry-based proteomics and microRNA sequencing. Our group has produced over 10 publications using these columns and we continue to be satisfied with the results. I would highly recommend incorporating the columns as part of the EV enrichment workflow for any researchers struggling with EV yield and purity."

Optimised For Your Research

qEV columns are available in an array of sizes to accommodate a wide range of biofluids and sample volumes.

High Purity

Gen 2 columns remove ~99% of soluble protein.

ISO 13485 Certified

Quality certified to ISO 13485: 2016 – Medical Devices

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