Rapid, High Precision
Extracellular Vesicle Isolation

Isolation of extracellular vesicles (EVs) is a challenge due to their small size, the complex biofluids in which they are often found, and the fragile nature of these vesicles. Izon’s qEV columns enable highly pure samples of intact EVs to be isolated from various fluids including saliva, complex biological milieu such as plasma, and cell culture supernatant. Based on size-exclusion chromatography, the columns offer rapid (<15 minute) and effective isolation into user-specified volumes and buffers.
Browse qEV Products
Automatic fraction collector (AFC) side on right
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Rapid, Simple & Reliable

qEV columns require minimal user intervention and intact EVs elute within 15 minutes. The reproducibility and reliability of results have been confirmed in an inter-laboratory trial.
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Standardisable & Reproducible

Each column is stardardised, quality assured and certified to ISO 13485 standards (Medical Devices), making qEV isolation suitable for clinical research.
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Pure, Clean Isolation Samples

qEV columns provide clean and pure samples without affecting the structure and function of the EVs.
elution profile for a qEVoriginal column
Figure 1.
Typical elution profile for a qEVoriginal column with 0.5 mL of plasma loaded; proteins elute in a later volume than vesicles. The vesicle concentration was measured using a qNano and protein levels by absorbance at 280nm.

Rapid. Gentle. Precise.

qEV columns remove >97% of contaminating soluble proteins in most cases. The columns are compatible with most physiologically relevant buffers, meaning that high yields of EVs can be obtained in conditions that are suitable for electron microscopy, proteomics, RNA analysis, physical measurement, and other downstream analyses.

Optimised For Your Research

The rapidly growing qEV range now includes 10 different columns.

Pure

Removes > 99% of soluble proteins
Automatic fraction collector (AFC) front on

The new standard in exosome isolation

Prior to the advent of SEC columns, UC was regarded as the gold standard for EV concentration and isolation. UC is time consuming and cannot provide a consistent standardised sample. The very high forces in UC, up to 200,000g, affect, disrupt and aggregate the vesicles, which may in turn invalidate much of the research.

Moreover, aggregated proteins and nucleic acid contaminants are present in the pellet containing the EVs. Density gradient centrifugation (DGC) aims to improve the purity of UC derived EVs but increases the complexity and time of the procedure and also decreases the yield of EVs. Standardised sampling across different laboratories is not achievable with either UC or DGC. Precipitation reagent kits have been adapted for exosome/EV purification. They are typically PEG based and sediment a wide range of product, not just EVs. Published independent data indicates these fractions are heavily contaminated with non-EV material because these reagents cause co-precipitation of proteins, lipoproteins and other biological components. qEV SEC columns provide clean samples without affecting the structure or function of the EV. Precipitation reagent kits provide dirty samples that vary from batch to batch. The leading researchers in the EV field no longer use or recommend precipitation products.
ev isolation from sample

How qEV Isolation Works

qEV is based on size exclusion chromatography (SEC). Size exclusion chromatography uses a stationary phase consisting of porous resin particles. Molecules smaller than the isolation range (35nm+ or 70nm+) are slowed because they enter into the pores of the stationary phase. Larger particles which cannot enter the pores flow around the resin and are eluted from the column earlier.

qEV - Now Automated & Highly Scalable

The world’s first automated, smart EV isolation system is here. A significant enhancement to the qEV platform, the Izon Automatic Fraction Collector (AFC) allows for fast, precise, automated isolation of EVs. Until now the qEV process has been labour intensive and difficult to scale. The AFC automates the qEV isolation process, eliminating human error, enabling high-precision large-scale, standardisable exosome isolation. A new era of EV isolation is here.
Learn more
Automatic fraction collector (AFC) side on right

Choose qEV Column Optimised For Your Research

There are currently 5 different Izon columns sizes to choose from, each available in 2 isolation ranges (designated 35 or 70). As each column has its own advantages.

Choose Your Column Size

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There are five qEV column sizes to choose from: qEVsingle, qEVoriginal, qEV2, qEV10 and qEV100.
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qEV single column
qEVsingle
< 150 µL
Sample loading (recommended for highest purity).
Ideal for clinical samples, RT-PCR
Optimised for small samples. No RNA carryover (single use). Compatible with the Automatic Fraction Collector.
Single use
qEV original column
qEVoriginal
< 500 µL
Sample loading (recommended for highest purity).
Ideal for higher volume research
The original and most popular qEV column. Compatible with the Automatic Fraction Collector.
Reusable
Up to 5 times.
qEV2 column
qEV2
<2 ml
Sample loading (recommended for highest purity).
Ideal for larger clinical samples and preparation for RNA analysis
Includes double Leur Lock fitting. Compatible with the Automatic Fraction Collector
Reusable
Up to 5 times.
qEV10 column
qEV10
<10 ml
Sample loading (recommended for highest purity).
Ideal for large volume cell culture supernatant
Includes double Leur Lock fitting. Compatible with the Automatic Fraction Collector.
Reusable
Up to 5 times.
qEV 100 column
qEV100
< 100 ml
Sample loading (recommended for highest purity).
Ideal for large scale, fast EV isolation
Includes double Leur Lock fitting.
Reusable
Up to 5 times.

Choose Your Isolation Range

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Each of the 5 different column sizes are now available in two isolation ranges (35nm+ and 70nm+). The popular 70nm+ qEV columns have an optimum recovery of particles from 70nm to 1000nm, while the newer 35nm+ columns have an optimum recovery range of 35nm to 350nm.
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