Rapid, High Precision
Extracellular Vesicle Isolation

Isolation of nanoparticles such as extracellular vesicles (EVs) is a challenge due to the complex biofluids in which they are found, their small size, and membranous structure which can be disrupted by isolation or handling factors. Izon’s qEV isolation columns enable highly purified samples of intact EVs to be isolated simply and with ease from a wide variety of fluids, such as saliva, plasma, and bronchoalveolar lavage fluid. By harnessing principles of size exclusion chromatography qEV columns offer rapid (<15 minute) and effective isolation into user-specified volumes and buffers.
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Automatic fraction collector (AFC) side on right
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Rapid, Simple & Reliable Isolation

qEV columns elute intact EVs within 15 mins and require minimal user intervention. The reproducibility and reliability of results have been confirmed in an inter-laboratory trial.
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Standardisable & Reproducible Results

Each column is stardardised, quality assured and certified to the ISO 13485 Medical Device standard, making qEV isolation suitable for clinical research.
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Pure, Intact & Functional EV Collection

qEV columns provide highly purified samples of intact EVs.
Figure 1.
Eluted protein and extracellular vesicles (EVs) and similarly sized particles >60 nm in human plasma (0.5 mL loading volume) separated on qEVoriginal/35 nm Gen 2 and qEVoriginal/70 nm Gen 2 columns. EV concentration was measured using an Exoid and protein levels by bicinchoninic acid (BCA) assay. Faded bars represent calculated individual concentrations based off pooled sample measurements.
*Volumes are labelled as the highest volume in that sample i.e label “0.4” refers to the volume from 0.0-0.4 mL after the buffer volume, label “0.8” refers to the volume from 0.4-0.8 mL after the buffer volume and so on.

Rapid, Gentle & Precise EV Isolation

qEV columns remove over 99% of contaminating soluble proteins and ensure that a high yield of EVs remain. EVs can then be studied using a range of techniques such as tunable resistive pulse sensing, electron microscopy, proteomics analysis and RNA analysis. As the columns in our qEV range are compatible with most physiologically relevant buffers, high yields of EVs can be obtained from a wide range of biological fluids.
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How qEV Isolation Columns Work

qEV isolation is based principles of on size exclusion chromatography which separates molecules and particles by size as they pass through a column consisting of porous resin particles. Larger particles elute the earliest, as they cannot enter the pores and therefore flow around the resin. In contrast, particles smaller than the isolation range (35 nm+ or 70 nm+) enter pores in the resin and elute later.
ev isolation from sample

The New Standard in EV Isolation

Before size exclusion chromatography was introduced, ultracentrifugation (UC) was regarded as the gold standard for EV isolation. However, as qEV columns provide clean, intact samples size exclusion chromatography has rapidly become the preferred technique. Having a higher quality sample means that downstream analysis will be easier and more reliable.

In contrast to UC, which is time-consuming and can't be standardised, the qEV platform is quick to run and can be standardised across different laboratories. UC uses high forces which can disrupt and aggregate particles and the resulting pellet may contain contaminants such as proteins and nucleic acid. While density gradient centrifugation (DGC) aims to improve UC's process of isolating EVs t increased the analytical time and complexity while decreasing EV yield. As a result standardised sampling across different laboratories is not achievable with either UC or DGC.

Precipitation reagent kits have also been adapted for EV purification, however they are typically PEG-based and sediment a wide range of contaminants along with EVs. Published, independent data indicates these fractions are heavily contaminated with non-EV material because these reagents cause co-precipitation of proteins, lipoproteins and other biological components. For these reasons, there is significant batch-to-batch variability, so precipitation products are no longer recommended within the EV field.

qEV Isolation is Now Automated & Highly Scalable

Introducing the world’s first automated, smart EV isolation system, the Automatic Fraction Collector (AFC). A significant enhancement to the qEV platform, the AFC allows fast, precise, automated isolation of EVs. Until now the qEV process has been labour-intensive and difficult to scale. By automating the qEV isolation process, the AFC minimises human error while enabling high-precision, large scale, standardisable EV isolation.
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Automatic fraction collector (AFC) side on right
Figure 3.
Legacy vs Gen 2. Data shown for a human plasma sample collected on qEV original columns, 0.5 mL loading volume.

Introducing qEV Gen 2

The new range of qEV columns are made with a proprietary, agarose resin, which delivers a more purified extracellular vesicle (EV)-containing eluate. The release of Gen 2 qEV columns are in line with the need to support the rapidly growing areas of EV research and applications, where sample purity has a huge impact on results downstream.
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Choose a qEV Isolation Column Optimised for Your Research

We have a range of qEV isolation columns suited to different size ranges and sample volumes to suit your research needs. Follows the steps below to select the right column for you.

Step 1 / Choose Your Column Size

Column size selection is based on the sample loading required. Each column has a sample loading volume recommend for highest purity. If you are not sure which column is the right size, please contact the Izon team.
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qEVsingle
< 150 µL
Sample loading (recommended for highest purity).
Ideal for clinical samples, RT-PCR
Optimised for small samples. No RNA carryover (single use). Compatible with the AFC.
Single use
Available Resins
Legacy only.
qEVoriginal
< 500 µL
Sample loading (recommended for highest purity).
Ideal for higher volume research
The original and most popular qEV column. Compatible with the AFC.
Reusable
Up to 5 times.
Available Resins
Gen 2 & Legacy.
qEV1
< 1 ml
Sample loading (recommended for highest purity).
Ideal for clinical samples and preparing for EV-RNA analysis
The qEV1 is our latest column made with our new proprietary resin. Compatible with the AFC.
Reusable
Up to 5 times.
Available Resins
Gen 2 only.
qEV2 column
qEV2
<2 ml
Sample loading (recommended for highest purity).
Ideal for larger clinical samples and preparation for RNA analysis
Includes double Leur Lock fitting. Compatible with the AFC.
Reusable
Up to 5 times.
Available Resins
Legacy only.
qEV10 column
qEV10
<10 ml
Sample loading (recommended for highest purity).
Ideal for large volume cell culture supernatant
Includes double Leur Lock fitting. Compatible with the AFC.
Reusable
Up to 5 times.
Available Resins
Legacy only.
qEV 100 column
qEV100
< 100 ml
Sample loading (recommended for highest purity).
Ideal for large scale, fast EV isolation
Includes double Leur Lock fitting.
Reusable
Up to 5 times.
Available Resins
Legacy only.

Step 2 /Choose Your Isolation Range

Each of the column sizes are available in two isolation ranges (35 nm and 70 nm). The 70 nm qEV columns have an optimum recovery of particles from 70 nm to 1000nm, while the newer 35 nm columns have an optimum recovery range of 35 nm to 350 nm.
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Optimised For Your Research

The rapidly growing qEV range now includes columns in a range of sizes, resins and isolation ranges.

Pure

Trials have shown that qEV columns remove more than 99% of soluble proteins.
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