The differences between the 35 nm and 70 nm Series stem from the size of the pores in the agarose resin. Compared to the qEV 70 nm Series, the qEV 35 nm Series has a smaller resin pore size. As a result, fewer small particles are slowed by the 35 nm resin, leading to a higher recovery of small particles (including EVs at the small end of the size spectrum) by 35 nm columns. However, this also results in more contaminating protein being present in isolated samples, relative to the 70 nm Series. For context though, both ranges of Gen 2 qEV columns remove ~99% contaminating protein. qEV 35 nm columns have an optimal recovery range of 35 nm – 350 nm, and the qEV 70 nm columns have an optimal recovery range of 70 nm – 1000 nm. If you would like more guidance on which qEV column range is right for your research, get in touch
and one of our team members will be able to assist.