Transform your research with precision and accuracy
Measure individual particle size, concentration, and charge, with unparalleled precision and accuracy. Tunable Resistive Pulse Sensing (TRPS) measures nanoparticles suspended in electrolytes on a particle-by-particle basis as they pass through a nanopore. This is a huge step forward as the more commonly used light scattering techniques only provide bulk estimates with low accuracy and very low precision. Used in over 45 countries, and included in over 900 publications, TRPS instruments are a key requirement for analysing nanoparticles scientifically and correctly.
Measure individual particles, with sub-nm precision.
The precision, resolution, and accuracy of size measurement in TRPS is unmatched by any other nanoparticle analysis technique. The transient current pulse caused by a particle traversing the pore is directly proportional to particle volume, enabling a highly precise and repeatable measurement of size to be achieved; in ideal conditions TRPS offers sub nm resolution of diameter. Every particle measured is compared to a set of NIST traceable calibration particles of known size, which ensures accuracy and repeatability. This calibration step enables the real size distribution of a particle set to be generated to a very high precision and accuracy. The measurement of particle concentration is also the best available, with data accuracy on a linear scale. By way of comparison, DLS is unable to measure concentration at all, and NTA is only accurate on a log scale and is too coarse for most scientific purposes.
Simultaneous size and concentration measurement
With TRPS users can simultaneously measure particle size and concentration. As particles individually pass through a nanopore it creates a blockade, the magnitude of the blockade is directly proportional to particle size while the frequency of the blockades is used to determine concentration. This is important in fields such as extracellular vesicle investigation as particles are typically heterogeneous. A standardised approach, such as TRPS, to measuring them is required for accurate, replicable, and precise data.
The Exoid is capable of measuring heterogenous samples over a wide size distribution, without the need for multiple measurements or adjustment of settings. Subpopulations can be identified with high resolution and accurately quantified individually.
Single-particle zeta potential measurement
The unique capability of TRPS to simultaneously measure particle size and zeta potential (which is a measure of surface charge) reproducibly on a particle-by-particle basis with high precision and accuracy presents a new and powerful approach for the life sciences. In comparison, PALS is an ensemble zeta potential measurement technique which is less precise than single particle measurement. PALS is also unable to measure concentration.
The Exoid is the next generation of TRPS device. The Exoid has the proven quality TRPS technology developed with the previous generation, qNano, but significantly improves the user experience making TRPS measurement easier than ever before.