Izon Science has announced the launch of Gen 2, the enhanced range of qEV columns for size-exclusion chromatography-based separation. The new range of qEV columns are made with a proprietary, agarose resin, which delivers a more purified extracellular vesicle (EV)-containing eluate. The release of Gen 2 qEV columns are in line with the need to support the rapidly growing areas of EV research and applications, where sample purity has a huge impact on results downstream.
The proprietary resin used in Gen 2 columns enables a greater proportion of protein to be removed from loaded samples, as shown in Figure 1:
The enhanced sample purity of Gen 2 columns can also be demonstrated by comparing the EV-to-protein ratio. The number of EVs was obtained via the Exoid, Izon’s tunable resistive pulse sensing (TRPS) nanoparticle measurement system which enables analysis on a particle-by-particle basis. Figure 2 shows separation capabilities in Legacy versus Gen 2 columns:
The enhanced separation of Gen 2 columns provides more purified EV-containing samples, which is highly desirable for downstream analysis and functional studies.
There is also another major benefit to the increased purity seen across purified collection volumes (PCVs). The improved separation provides a new level of control when deciding which PCVs to pool. As shown below, the composition of pooled volumes can be influenced by selecting different PCVs:
Following column optimisation, an additional benefit became apparent: the Gen 2 qEVoriginal/70 nm column delivers a higher concentration of EVs, when compared to its Legacy counterpart (the Legacy qEVoriginal/70 nm).
The first Gen 2 columns to be released are the qEVoriginal Gen 2, and the qEV1 – a column optimised for a 1 mL loading volume. Both are available now in the existing 35 nm and 70 nm series, which have optimum recovery ranges of 35-350 nm and 70-1000 nm, respectively. The qEV1 is a valuable addition for the many researchers working with lower sample volumes, particularly those with interests in EV-RNA and biomarker discovery and development.
Since the first launch of the qEVoriginal in 2014, the existing range of Legacy columns has expanded to suit different sample loading volumes, including the qEVsingle (2016), qEV2 and qEV10 (2018) and the qEV100 (2019). qEV columns have since been used in studies of diverse sample types including plasma, cell culture supernatant, cerebrospinal fluid, and bronchoalveolar lavage fluid. The introduction of the Automatic Fraction Collector (AFC) in 2019 marked a major shift from labour-intensive manual collection to a rapid and reproducible method that can be scaled up for larger-scale biomarker studies. The release of the Gen 2 columns reflects Izon’s commitment to providing a scalable way forward for EV applications, including EV-diagnostics.