qEV2 Gen 2 Available Now: Isolate EVs from 2 mL Sample Loading Volumes
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The qEV2 Gen 2 column is the sixth column to join the Gen 2 range; launched on 17th Jan 2023.
The qEV2 Gen 2 column, a size exclusion chromatography column optimised for the isolation of extracellular vesicles (EVs) from 2 mL sample loading volumes, is now available.
The Gen 2 qEV range now includes columns of six different sizes, each optimised for different sample loading volumes from 150 µL – 100 mL.
Gen 2 qEV columns are made with an exclusive resin which enables EVs to be isolated to a high level of purity. The performance of qEV2 Gen 2 columns has been assessed via measures of EVs and protein, as shown in the figures below.
Maximise EV purity with qEV2 Gen 2
Figure 1. Number of extracellular vesicles (EVs) larger than 60 nm (measured using the Exoid) per microgram of protein (measured using the bicinchoninic acid assay) isolated from human plasma samples with Legacy and Gen 2 qEV2 columns. EVs were isolated using the Automatic Fraction Collector and default buffer volumes established for qEV2 Gen 2 columns. Left: 35 nm Series; right: 70 nm Series.
Figure 2. Protein present in pooled collection volumes (8 mL) of human plasma separated using qEV2 columns. Protein was measured via bicinchoninic acid (BCA) assay before and after extracellular vesicles (EVs) were isolated using the Automatic Fraction Collector and default buffer volumes established for qEV2 Gen 2 columns. Left: 35 nm Series; right: 70 nm Series.
Like all other qEV columns, the qEV2 Gen 2 is available in the 35 nm Series and 70 nm Series. You can shop for qEV2 Gen 2 columns online or by contacting our team for a quote.
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Written by alex@kholab.co, last updated 14/9/21
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