Izon Science has introduced a new qEV column optimised for 1 mL sample loading volumes: the qEV1.
The new column is built with a proprietary, agarose resin which delivers improved separation, compared to the resin used in Izon’s existing range of Legacy columns.
The qEV1 was developed to meet the strong demand for a column optimised for a 1 mL sample – a common sample volume obtained in clinical studies, general research, EV biomarker development, and sample consortiums.
Previously, those with 1 mL samples at hand would overload qEVoriginal columns, which are designed to support 0.5 mL loading volumes. Doing so would lead to sub-optimal EV-protein separation. Alternatively, loading a 1 mL sample onto a qEV2 creates unnecessary sample dilution.
Ultimately, the creation of the qEV1 was driven by this strong need for a 1 mL column – and was made possible by the recent development of the novel, proprietary resin used in Gen 2 qEV columns.
Selecting the most optimal column size for your sample is important for maximising the recovery and purity of EVs, as it helps maximise the signal-to-noise ratio in analyses downstream.
The figures below illustrate the improved purity that EV researchers can expect from the qEV1 (1 mL loading volume) compared to a qEVoriginal (Legacy column, 1 mL loading volume).
The qEV1 bridges the gap that previously existed between the qEVoriginal and qEV2, and has been fully optimised with the Gen 2 resin to deliver an enhanced level of purity.
Together with the automation and reproducibility provided by Izon’s Automatic Fraction Collector, the qEV1 provides a highly effective method for isolating EVs from 1 mL loading volumes.
The novel resin used in the qEV1 is included in all Gen 2 columns, the first of which were launched in mid-October 2021. The Gen 2 range is available across the same recovery ranges as before: 35 to 350 nm (the 35 nm+ series) and 70 to 1000 nm (70 nm+ series).