Metabolomic Analysis of Extracellular Vesicles from Human Synovial Fluids

Extracellular Vesicles

Chen, Daosen, Huachen Yu, Yu Zhang, and Yijiang Huang. 2022. “Metabolomic Analysis of Extracellular Vesicles from Human Synovial Fluids.” Microchemical Journal 177 (June): 107257.

Osteoarthritis (OA) is a highly prevalent type of joint disease while the pathological mechanisms behind the disease development are not completely known yet. Extracellular vesicles (EVs) participating in various physiological and pathological processes have increasingly attracted attentions for their potential roles in inflammatory arthritis. In this work, we performed lipidomic analysis of EVs from human synovial fluids using three most applied EV isolation methods including ultracentrifugation (UC), polyethylene glycol mediated precipitation method (PEG) and size-exclusive chromatography (SEC). The lipidomic profiles of synovial EVs were then determined by UHPLC-MS technique targeting 12 classes of lipids with lipid standards. Further comparative analysis shows that the PEG method identified the most lipids but lipoprotein contamination was observed. The purity of EV products from UC was the highest as showing the least lipoprotein enriched lipids. Thus, UC might be the most suitable method for synovial EV lipidomic analysis. Investigation of synovial EVs from OA patients provides unique insights of OA initiation and maintenance, and the dysregulated EV-associated molecules might be potentially employed as novel diagnostic and therapeutic targets.

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