Following the release of the qEV1 in mid-October 2021, we share answers to questions you may have.
The qEV1 is a column which separates particles using size-exclusion chromatography. Column dimensions and resin volume have been optimised for the separation of 1 mL sample loading volumes.
Most ‘raw’ samples cannot be directly run on qEV columns without prior preparation steps. Once your sample has been appropriately prepared, then you can load 1 mL. Visit the Izon Science Support Centre for recommendations and protocols.
Selecting the most optimal column size for your sample is important for maximising the purity of EVs in your sample.
Many wanted a column that was optimised for 1 mL loading volumes – particularly those working with EV-RNA. Previously, however, our low-volume columns were only available for 150 µL (the qEVsingle), 0.5 mL (the qEVoriginal), and 2 mL (the qEV2) loading volumes.
The qEVoriginal is designed to support 0.5 mL loading volumes. If you load 1 mL onto a qEVoriginal, EV-protein separation will be suboptimal. This means that the separated volumes will contain a lot more protein than would be the case if you used a qEV1.
Again, separation will be suboptimal. You will be diluting your sample more than is necessary.
Two reasons: as explained above, the qEV1 has been optimised for a 1mL loading volume, whereas the qEVoriginal is for 0.5 mL. Also, the qEV1 uses a novel agarose resin which provides better separation than our existing Legacy columns.
The qEV1 is 1.8 cm taller (0.7 inches) than the Legacy qEVoriginal, as the column dimensions have been optimised to cater to the 1 mL sample volume using the new resin.
Gen 2 columns are built with an agarose resin. It performs a lot better than the resin used in our existing Legacy columns. The qEV1 joins the novel Gen 2 qEV range, alongside the Gen 2 versions of the qEVoriginal.