Rapid and Precise
Extracellular Vesicle Isolation

Reproducible results require reproducible isolation, but not all methods are created equal. While a range of techniques can be used to isolate exosomes and other extracellular vesicles (EVs), many are irreproducible, laborious, and/or offer low levels of EV purity and recovery. qEV columns, however, harness size exclusion chromatography to separate EVs from soluble protein and deliver highly purified EVs in an efficient and standardised manner. qEV columns are available in an array of sizes, allowing rapid separation to be achieved for a wide range of biofluids and sample volumes.
Browse qEV columns

Isolate EVs in minutes, not hours

Forget centrifugation marathons - EVs elute from qEV columns within 15 minutes.

Simple & reproducible

Consistently and easily separate EVs from soluble proteins with qEV columns. Combine with the Automatic Fraction Collector to enhance reproducibility and ease of use.

A gentle approach to EV isolation

qEV columns offer a gentle approach to isolation, allowing EVs to remain intact.
Figure 1.
Eluted protein and extracellular vesicles (EVs) and similarly sized particles >60 nm in human plasma (0.5 mL loading volume) separated on qEVoriginal/35 nm Gen 2 and qEVoriginal/70 nm Gen 2 columns. EV concentration was measured using an Exoid and protein levels by bicinchoninic acid (BCA) assay. Faded bars represent calculated individual concentrations based off pooled sample measurements.
*Volumes are labelled as the highest volume in that sample i.e label “0.4” refers to the volume from 0.0-0.4 mL after the buffer volume, label “0.8” refers to the volume from 0.4-0.8 mL after the buffer volume and so on.

Isolate Highly Purified Extracellular Vesicles

qEV columns are widely used in the extracellular vesicle (EV) field, for reasons that extend beyond their ease of use and gentle, efficient approach. qEV columns use principles of size exclusion chromatography (SEC) to separate contaminating soluble protein from exosomes and other EVs. Izon’s Gen 2 qEV columns remove approximately 99% of soluble protein – delivering even more highly purified samples than was possible with the existing range (Legacy qEV columns). As the co-isolation of non-EV components impacts downstream applications and analysis, the question of purity in EV isolation is an important one. Following qEV isolation, EVs can then be studied using a range of techniques including tunable resistive pulse sensing, electron microscopy, proteomics and RNA analysis.
View qEV range

How qEV Isolation Columns Work

qEV isolation is based on principles of size exclusion chromatography, a method in which molecules and particles in solution are separated by their size as they pass through a column consisting of porous resin particles. In qEV isolation, larger particles are unable to enter pores in the resin, and instead flow through the resin relatively quickly. In contrast, particles smaller than the lower end of the isolation range (35 nm or 70 nm) enter pores in the resin, which slows their journey. Through this size-based separation, EVs and soluble protein are separated to a high degree of resolution, and a purified volume can be obtained.

qEV Isolation is Now Automated & Highly Scalable

The AFC automates extracellular vesicle (EV) isolation and enables precise and reproducible separation. Previously, the process of EV isolation has been labour-intensive and difficult to scale. Using the AFC, together with qEV size exclusion chromatography columns, EV isolation can be achieved easily and with minimal intervention.
Learn more about the AFC

Introducing qEV Gen 2

The new range of qEV columns are made with an agarose resin, which delivers a more purified extracellular vesicle (EV)-containing eluate. The release of Gen 2 qEV columns are in line with the need to support the rapidly growing areas of EV research and applications, where sample purity has a huge impact on results downstream.
Learn more about qEV Gen 2
Figure 3.
Legacy vs Gen 2. Data shown for a human plasma sample collected on qEV original columns, 0.5 mL loading volume.

Choose a qEV Isolation Column Optimised for Your Research

To meet your research needs, we have a range of qEV Isolation Columns suited to different particle size isolation ranges and sample volumes.

When selecting a qEV column, consider the ideal purified collection volume (PCV) you require for downstream analysis, the sample loading volume, and how much contaminating lipoprotein overlap is acceptable.

To select the most appropriate column for your research follow these two steps:

Step 1 / Choose Your Column Size

Column size selection is based on the sample loading volume required. Each column has a sample loading volume recommended for highest purity. If you are not sure which column is the right size, please contact the Izon team.

Izon products are designed and manufactured under a quality system certified to ISO 13485:2016.
arrow down
qEVsingle Gen 2 70 nm
150 µL
Sample loading (recommended for highest purity).
Ideal for small biological samples
Optimised for small samples. Compatible with the AFC.
Single use
No RNA carryover (single use).
qEVoriginal Gen 2 70 nm
500 µL
Sample loading (recommended for highest purity).
Ideal for high-throughput studies
The original and most popular qEV column. Compatible with the AFC.
Up to 5 times.
qEV1 Gen 2 70 nm
1 mL
Sample loading (recommended for highest purity).
Ideal for high-throughput studies and EV-RNA preparation
The qEV1 is our latest column made with our new Gen 2 resin. Compatible with the AFC.
Up to 5 times.
2 mL
Sample loading (recommended for highest purity).
Ideal for larger clinical samples and preparation for RNA analysis
Includes double Leur Lock fitting. Compatible with the AFC.
Up to 5 times.
qEV1 Gen 2 70 nm Column
10 mL
Sample loading (recommended for highest purity).
Ideal for large volume cell culture supernatant
Includes double Leur Lock fitting. Compatible with the AFC.
Up to 5 times.
100 mL
Sample loading (recommended for highest purity).
Ideal for industrial volumes of cell culture supernatant
Includes double Leur Lock fitting.
Up to 5 times.
arrow pointing right
arrow pointing right

Step 2 /Choose Your Isolation Range

All column sizes are available in two isolation ranges. The popular 70 nm qEV columns have an optimum particle recovery range of 70 nm to 1000 nm, while the 35 nm columns have an optimum recovery range of 35 nm to 350 nm. The 70 nm Series columns have less protein overlap with EVs, whereas the 35 nm Series columns provide a higher recovery of EVs that are smaller than 110 nm.
arrow down

qEV Concentration Kit and qEV RNA Extraction Kit

Impactful extracellular vesicle (EV) research and the development of downstream applications are only possible when supported by technologies that standardise and maximise the purification of EV and EV-RNA samples.

To support your downstream applications, Izon offers two kits: the qEV Concentration Kit for concentrating EV samples, and the qEV RNA Extraction Kit, which enables the extraction of RNA from EVs.  
Learn more about the qEV kits
Natalie Turner, PhD Student at Queensland University of Technology
"Izon’s qEV columns offer a simple, effective and reproducible way of purifying EVs directly from biofluids or from EV enriched samples following differential centrifugation. We use the qEV 70 nm legacy (original) columns for all of our EV preps from plasma and milk samples, which undergo downstream processing for mass spectrometry-based proteomics and microRNA sequencing. Our group has produced over 10 publications using these columns and we continue to be satisfied with the results. I would highly recommend incorporating the columns as part of the EV enrichment workflow for any researchers struggling with EV yield and purity."

Optimised For Your Research

qEV columns are available in an array of sizes to accommodate a wide range of biofluids and sample volumes.

High Purity

Gen 2 columns remove ~99% of soluble protein.

Start a Conversation About Your Research


Latest Articles & News

View All >