Rapid, High Precision
Extracellular Vesicle Isolation

Isolation of nanoparticles such as extracellular vesicles (EVs) is a challenge due to the complex biofluids in which they are found, their small size, and membranous structure which can be disrupted by isolation or handling factors. Izon’s qEV isolation columns enable highly purified samples of intact EVs to be isolated simply and with ease from a wide variety of fluids, such as saliva, plasma, and bronchoalveolar lavage fluid. By harnessing principles of size exclusion chromatography qEV columns offer rapid (<15 minute) and effective isolation into user-specified volumes and buffers.
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Rapid, Simple & Reliable Isolation

qEV columns elute intact EVs within 15 minutes and require minimal user intervention.
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Standardisable & Reproducible Results

The qEV isolation platform, which consists of the Automatic Fraction Collector and qEV columns, minimises manual error by providing an element of automation.
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Pure, Intact & Functional Extracellular Vesicle Collection

qEV columns provide highly purified samples of intact EVs, which is particularly important for functional studies
qEVoriginal ev elution profile without figure legend
Figure 1.
Eluted protein and extracellular vesicles (EVs) and similarly sized particles >60 nm in human plasma (0.5 mL loading volume) separated on qEVoriginal/35 nm Gen 2 and qEVoriginal/70 nm Gen 2 columns. EV concentration was measured using an Exoid and protein levels by bicinchoninic acid (BCA) assay. Faded bars represent calculated individual concentrations based off pooled sample measurements.
*Volumes are labelled as the highest volume in that sample i.e label “0.4” refers to the volume from 0.0-0.4 mL after the buffer volume, label “0.8” refers to the volume from 0.4-0.8 mL after the buffer volume and so on.

Rapid, Gentle & Precise Extracellular Vesicle Isolation

qEV columns remove over 99% of contaminating soluble proteins and ensure that a high yield of extracellular vesicles (EVs) remain. EVs can then be studied using a range of techniques such as tunable resistive pulse sensing, electron microscopy, proteomics analysis and RNA analysis. As the columns in our qEV range are compatible with most physiologically relevant buffers, high yields of EVs can be obtained from a wide range of biological fluids.
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How qEV Isolation Columns Work

qEV isolation is based principles of on size exclusion chromatography which separates molecules and particles by size as they pass through a column consisting of porous resin particles. Larger particles elute the earliest, as they cannot enter the pores and therefore flow around the resin. In contrast, particles smaller than the isolation range (35 nm+ or 70 nm+) enter pores in the resin and elute later.
ev isolation from sample

The New Standard in Extracellular Vesicle Isolation

Before size exclusion chromatography was introduced, ultracentrifugation (UC) was regarded as the gold standard for extracellular vesicle (EV) isolation. However, as qEV columns provide clean, intact samples size exclusion chromatography has rapidly become the preferred technique. Having a higher quality sample means that downstream analysis will be easier and more reliable.

In contrast to ultracentrifugation, which is time-consuming and can't be standardised, the qEV platform is quick to run and can be standardised across different laboratories. Ultracentrifugation uses high forces which can disrupt and aggregate particles and the resulting pellet may contain contaminants such as proteins and nucleic acid. While density gradient centrifugation aims to improve ultracentrifugation's process of isolating EVs t increased the analytical time and complexity while decreasing EV yield. As a result standardised sampling across different laboratories is not achievable with either ultracentrifugation or density gradient centrifugation.

Precipitation reagent kits have also been adapted for EV purification, however they are typically PEG-based and sediment a wide range of contaminants along with EVs. Published, independent data indicates these fractions are heavily contaminated with non-EV material because these reagents cause co-precipitation of proteins, lipoproteins and other biological components. For these reasons, there is significant batch-to-batch variability, so precipitation products are no longer recommended within the EV field.

qEV Isolation is Now Automated & Highly Scalable

The AFC automates extracellular vesicle(EV) isolation and enables precise and reproducible separation. Previously, the process of EV isolation has been labour-intensive and difficult to scale. Using the AFC, together with qEV size exclusion chromatography columns, EV isolation can be achieved easily and with minimal intervention.
Learn more about the AFC
qEVoriginal EVs per mircogram of protein comparison
Figure 3.
Legacy vs Gen 2. Data shown for a human plasma sample collected on qEV original columns, 0.5 mL loading volume.

Introducing qEV Gen 2

The new range of qEV columns are made with a proprietary, agarose resin, which delivers a more purified extracellular vesicle (EV)-containing eluate. The release of Gen 2 qEV columns are in line with the need to support the rapidly growing areas of EV research and applications, where sample purity has a huge impact on results downstream.
Learn more about Gen 2 qEV

Choose a qEV Isolation Column Optimised for Your Research

To meet your research needs, we have a range of qEV Isolation Columns suited to different particle size isolation ranges and sample volumes.

When selecting a qEV column, consider the ideal purified collection volume (PCV) you require for downstream analysis, the sample loading volume, and how much contaminating protein overlap is acceptable.

To select the most appropriate column for your research follow these two steps:

Step 1 / Choose Your Column Size

Column size selection is based on the sample loading volume required. Each column has a sample loading volume recommended for highest purity. If you are not sure which column is the right size, please contact the Izon team.

Izon products are designed and manufactured under a quality system certified to ISO 13485:2016.
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qEVsingle Gen 2 70 nm
qEVsingle
< 150 µL
Sample loading (recommended for highest purity).
Ideal for small biological samples
Optimised for small samples. Compatible with the AFC.
Single use
No RNA carryover (single use).
Available Resins
Gen 2 & Legacy.
qEVoriginal Gen 2 70 nm
qEVoriginal
< 500 µL
Sample loading (recommended for highest purity).
Ideal for high-throughput studies
The original and most popular qEV column. Compatible with the AFC.
Reusable
Up to 5 times.
Available Resins
Gen 2 & Legacy.
qEV1 Gen 2 70 nm
qEV1
< 1 ml
Sample loading (recommended for highest purity).
Ideal for high-throughput studies and EV-RNA preparation
The qEV1 is our latest column made with our new proprietary resin. Compatible with the AFC.
Reusable
Up to 5 times.
Available Resins
Gen 2 only.
qEV2 70 nm Legacy column
qEV2
<2 ml
Sample loading (recommended for highest purity).
Ideal for larger clinical samples and preparation for RNA analysis
Includes double Leur Lock fitting. Compatible with the AFC.
Reusable
Up to 5 times.
Available Resins
Legacy only.
qEV1 Gen 2 70 nm Column
qEV10
<10 ml
Sample loading (recommended for highest purity).
Ideal for large volume cell culture supernatant
Includes double Leur Lock fitting. Compatible with the AFC.
Reusable
Up to 5 times.
Available Resins
Gen 2 & Legacy.
qEV100
< 100 ml
Sample loading (recommended for highest purity).
Ideal for industrial volumes of cell culture supernatant
Includes double Leur Lock fitting.
Reusable
Up to 5 times.
Available Resins
Gen 2 & Legacy.
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Step 2 /Choose Your Isolation Range

All column sizes are available in two isolation ranges. The popular 70 nm qEV columns have an optimum recovery of particles from 70 nm to 1000 nm, while the 35 nm columns have an optimum recovery range of 35 nm to 350 nm.
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Optimised For Your Research

The rapidly growing qEV range now includes columns in a range of sizes, resins and isolation ranges.

Pure

Trials have shown that qEV columns remove more than 99% of soluble proteins.

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