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Direct and label-free influenza virus detection based on multisite binding to sialic acid receptors

Horiguchi, Yukichi, Tatsuro Goda, Akira Matsumoto, Hiroaki Takeuchi, Shoji Yamaoka, and Yuji Miyahara. "Direct and label-free influenza virus detection based on multisite binding to sialic acid receptors." Biosensors and Bioelectronics 92 (2017): 234-240.

A system to discriminate human or avian influenza A remains a highly sought-after tool for prevention of influenza pandemics in humans. Selective binding of the influenza A viral hemagglutinin (HA) to specific sialic acid (SA) receptors (Neu5Acα(2-6)Gal in humans, Neu5Acα(2-3)Gal in birds) is determined by the genotype of the HA and neuraminidase (NA) segments, making it one of the key characteristics that distinguishes human or avian influenza A virus. Here we demonstrate the direct detection of whole H1N1 influenza A virus using 6′-sialyllactose (Neu5Acα(2-6)Galβ(1-4)Glc, 6SL)-immobilized gold electrodes as biosensing surfaces. The sensitivity was higher than that of conventional immunochromatographic technique (ICT) for influenza virus and not restricted by genetic drift. The label-free detection technology via direct attachment of a whole virus using a chemically modified electrode is a promising means to provide a simple and rapid diagnostic system for viral infections.

Keywords

Biosensor

Biointerface

Influrenza virus

Molecular recognition

Label-free

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