Isolation of Extracellular Vesicles from Plasma
Van Deun, J., et. al., “The impact of disparate isolation methods for extracellular vesicles on downstream RNA profiling,” Journal of Extracellular Vesicles, 2014;3:10.3402/jev.v3.24858. doi:10.3402/jev.v3.24858
Lobb, RJ, et al., “Optimized exosome isolation protocol for cell culture supernatant and human plasma”, Journal of Extracellular Vesicles, 2015;4:10.3402/jev.v4.27031. doi:10.3402/jev.v4.27031.
Extracellular vesicles (EVs) are important mediators of intercellular communication and are involved in the transmission of biological information between cells to regulate a broad range of biological processes. EVs contain proteins, mRNA, and miRNA representing the cell they are secreted from and there is increasing evidence that EVs play an important role in normal physiological processes, development, human disease, and viral infections. EVs are found in most biological fluids; plasma is a rich reservoir of vesicles that can be easily obtained by drawing a patient’s blood. However, plasma is also a complex biological sample; rich in protein, high- and low-density lipoproteins (HDL/LDL), salts, ions, nutrients and other particulates and these must be removed before EV analysis. Traditional methods to isolate EVs such as ultracentrifugation and chemical precipitation yield impure aggregated vesicles and are expensive in both time and equipment.1,2 Because of these challenges, there is a need for a rapid, simple, and cost-effective method to isolate EVs from plasma.