The rise of SEC: 3 Essential EV publications
Size Exclusion Chromatography (SEC) is rapidly becoming the gold standard in EV isolation, due to the speed, efficiency, very clean samples and reproducibility. Ultra-centrifugation (UC) is time-consuming and cannot provide a consistent standardised sample. Optimised for EVs and standardised for repeatability, Izon's qEV SEC columns are quickly becoming the isolation method of choice for EV researchers.
If you are interested in whether to adopt SEC and qEV, we suggest you read the following publications:
- Böing, A. N., Van Der Pol, E., Grootemaat, A. E., Coumans, F. A., Sturk, A., & Nieuwland, R. (2014). Single-step isolation of extracellular vesicles by size-exclusion chromatography. Journal of extracellular vesicles, 3.
- Lobb, R. J., Becker, M., Wen Wen, S., Wong, C. S., Wiegmans, A. P., Leimgruber, A., & Möller, A. (2015). Optimized exosome isolation protocol for cell culture supernatant and human plasma. Journal of extracellular vesicles, 4(1), 27031.
- Vanni, I., Alama, A., Grossi, F., Dal Bello, M. G., & Coco, S. (2017). Exosomes: a new horizon in lung cancer. Drug Discovery Today.
Note that since the first Major SEC-EV paper was published in 2014 (Boing et al), work and protocols have advanced considerably. The recently released qEVsingle for instance is targeted specifically at diagnostic development and clinical use.