VIRUSES & BACTERIA
Virus Quantitation & Analysis using TRPS
Tunable Resistive Pulse Sensing (TRPS) enables rapid determination of total viral titre (particles per mL) and stability assessment of viruses and viral aggregates in liquid suspension.
Analysis of thousands of viral particles usually takes less than 15 minutes. Key applications include characterization of viral particles for vaccine development and accurate analysis of vectors for gene therapy research.
Vaccine development requires stable preparations with information on the number of virus particles present and how many of them are capable of infecting cells. Infectivity assays can answer the latter question but accurate determination of total virus numbers is difficult. TRPS can be applied to accurately correlate the total number of virus particles to virus infectious units (IFU) when combined with standard titer analysis methods. Reliable and accurate measurement of viral aggregation is a further benefit of the system not available through alternative methods.
Gene Therapy Research
Regulatory agencies demand a wide range of tests on the safety and efficacy of gene therapy after the development of usable vectors and before approving clinical studies. There is a need to develop supportive technologies for gene transfer beyond actual vector research such as measurement of total viral particle concentration and viable viral titre. A wide variety of common gene therapy vectors including viral (e.g., adenovirus, lentivirus, granulovirus) and non-viral vectors (e.g., CaP/DNA/PEI hybrid particles, exosomes) have been measured using TRPS.